Plasmid

Part:BBa_K4669029

Designed by: Jonas Westphal   Group: iGEM23_Hamburg   (2023-10-12)


pET22b(+)_R9-ferritin

pET22b(+)_R9-ferritin is a N-terminal fusion of the nona-arginine (R9) cell penetrating peptide (BBa_K4669001) to ferritin heavy chain (H. sapiens) (BBa_K4669000) on the pET22b(+) expression vector (BBa_K4669020). R9 and ferritin are joined by a GS-linker (BBa_K4669003) to prevent potential steric hindrance of R9 by ferritin heavy chain. Under native conditions, ferritin heavy chain assembles into ferritin cages consisting of 24 subunits. The presence of R9 could thus confer cell penetrative properties to R9-ferritin cages. The pET22b(+) expression vector allows the IPTG inducible expression of R9-ferritin in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5660
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5660
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5272
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5660
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5660
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 3492
    Illegal NgoMIV site found at 3652
    Illegal NgoMIV site found at 5240
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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