Composite

Part:BBa_K4669025

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-07)


TAT3 eGFP

TAT3-eGFP is a N-Terminal fusion of the Trans-Activator of Transcription 3 (TAT3) cell penetrating peptide (cpp) (BBa_K4669017) to eGFP (BBa_K4669015). A HA-tag (BBa_K4669019) between these two sequences acts as both a linker and affinity purification tag. Additionally, a polyhistidine tag (BBa_K3113011) at the C-Terminus can be used for affinity purification, which can be removed by TEV protease cleavage through a upstream TEV protease recognition site (BBa_K1362453).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None