Plasmid_Backbone
pET22b(+)

Part:BBa_K4669020

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-07)


pET22b(+) for expression

The pET22b(+) expression vector from Novagen. It can be used for IPTG induced expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 5272
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 3492
    Illegal NgoMIV site found at 3652
    Illegal NgoMIV site found at 5240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None