Composite

Part:BBa_K4669014

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-06)


PylRs and Pyl tRNA CUA combination

Combination of the Pyl aminoacyl tRNA synthetase, the corrosponding Pyl tRNA, araBAD promoters and the rrnB terminator for an expression system in E. coli inducible by arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 316
    Illegal BamHI site found at 239
    Illegal BamHI site found at 2253
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1022
    Illegal NgoMIV site found at 1606
    Illegal AgeI site found at 74
    Illegal AgeI site found at 2088
    Illegal AgeI site found at 2483
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None