Coding

Part:BBa_K4669011

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-05)


Pyl aminoacyl-tRNA synthetase

The pyrrolysyl-tRNA synthetase (PylRS) from Methanosarcina mazei for the incorporation of fluorophenylalanine derivatives. PylRS loads the Mm-tRNA Pyl CUA (BBa_K1722004) with the fluorophenylalanine derivative.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 701
    Illegal NgoMIV site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None