Part:BBa_K4669010
R12 - Human heavy chain ferritin K88TAG
R12-Amber-ferritin is a N-terminal fusion of the dodeca-arginine (R12) cell penetrating peptide (BBa_K4669002) to ferritin heavy chain (H. sapiens) with a lysine 88 to amber codon substitution (BBa_K4669007). Both parts are joined by a GS-linker (BBa_K4669003) to prevent potential steric hindrance of R12 by ferritin heavy chain. Under native conditions, ferritin heavy chain assembles into ferritin cages consisting of 24 subunits.The presence of R12 could thus confer cell penetrative properties to R12-ferritin cages. The amber codon (UAG) is a stop codon that can be used for the incorporation of non-canonical amino acids during protein synthesis, which requires the supplementation of the used culture media with said amino acid as well as a helper plasmid encoding for the corresponding tRNA and aminoacyl-tRNA synthetase. The incorporated amber codon enables a multitude of use cases, e.g. click chemistry to add another small molecule or protein to the non-canonical amino acid residue.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 277
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 277
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 277
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 277
- 1000COMPATIBLE WITH RFC[1000]
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