Part:BBa_K4669008
TAT - Human Heavy Chain Ferritin K88TAG
TAT-Amber-ferritin is a N-terminal fusion of the Trans Activator of Transcription (TAT) cell penetrating peptide (BBa_K1202006) to ferritin heavy chain (H. sapiens) with a lysine 88 to amber codon substitution (BBa_K4669007). Both parts are joined by a GS-linker (BBa_K4669003) to prevent potential steric hindrance of TAT by ferritin heavy chain. Under native conditions, ferritin heavy chain assembles into ferritin cages consisting of 24 subunits.The presence of TAT could thus confer cell penetrative properties to TAT-ferritin cages. The amber codon (UAG) is a stop codon that can be used for the incorporation of non-canonical amino acids during protein synthesis, which requires the supplementation of the used culture media with said amino acid as well as a helper plasmid encoding for the corresponding tRNA and aminoacyl-tRNA synthetase. The incorporated amber codon enables a multitude of use cases, e.g. click chemistry to add another small molecule or protein to the non-canonical amino acid residue.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 268
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 268
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 268
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 268
- 1000COMPATIBLE WITH RFC[1000]
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