Composite
R12-Ftn

Part:BBa_K4669006

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-04)


R12-Ferritin

R12-ferritin is a N-terminal fusion of the dodeca-arginine (R12) cell penetrating peptide (BBa_K4669002) to ferritin heavy chain (H. sapiens) (BBa_K4669000). Both parts are joined by a GS-linker (BBa_K4669003) to prevent potential steric hindrance of R12 by ferritin heavy chain. Under native conditions, ferritin heavy chain assembles into ferritin cages consisting of 24 subunits. The presence of R12 could thus confer cell penetrative properties to R12-ferritin cages.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None