Coding

Part:BBa_K4658003

Designed by: Tanvi Garg   Group: iGEM23_Stockholm   (2023-10-07)


Anchor Peptide, LCI

The anchor peptide is LCI, polymer binding peptide, which was used for the binding of the fusion protein to polypropylene.

To facilitate the binding, we used the fusion construct of the anchor peptide(BBa_K4658003), with the linker peptide(BBa_K4658000), AviTag(BBa_K4658002), AviTag Spacer(BBa_K4658001), and EGFP (BBa_K3033009 ) or NLuc(BBa_K1159001) which binds to the polypropylene, which can be confirmed by measuring the fluorescence or luminescence after multiple washes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None