Coding

Part:BBa_K4657004

Designed by: Ying Zhang   Group: iGEM23_BJWZ-China   (2023-10-05)


Coding sequence of Fusion araBAD promoter with btuB and rrnB T2 terminator

ParaBAD system for induction of btuB expression by the arabinose operon 1-6 bp: Xba I site; 7-291 bp: araBAD promoter 315-2159: coding sequence of btuB 2160-2187 bp: rrnB T2 terminator 2188-2193 bp: Xho I site


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
    Illegal BamHI site found at 1604
    Illegal XhoI site found at 2188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1407
    Illegal AgeI site found at 80
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2094
    Illegal SapI site found at 62


[edit]
Categories
Parameters
None