Coding
Part:BBa_K4657004
Designed by: Ying Zhang Group: iGEM23_BJWZ-China (2023-10-05)
Coding sequence of Fusion araBAD promoter with btuB and rrnB T2 terminator
ParaBAD system for induction of btuB expression by the arabinose operon 1-6 bp: Xba I site; 7-291 bp: araBAD promoter 315-2159: coding sequence of btuB 2160-2187 bp: rrnB T2 terminator 2188-2193 bp: Xho I site
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
Illegal PstI site found at 1407 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1407
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 245
Illegal BamHI site found at 1604
Illegal XhoI site found at 2188 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
Illegal PstI site found at 1407 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
Illegal PstI site found at 1407
Illegal AgeI site found at 80 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2094
Illegal SapI site found at 62
[edit]
Categories
Parameters
None |