Coding

Part:BBa_K4657002

Designed by: Ying Zhang   Group: iGEM23_BJWZ-China   (2023-10-05)


Coding sequence of btuB.

BtuB is an outer membrane porin that mediates high affinity binding and TonB- dependent active transport of vitamin B12 (cyanocobalamin) across the outer membrane. This gene has been optimized and adapted to our chassis strain E. coli MG1655 at the time of synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1093
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1290
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1780


[edit]
Categories
Parameters
None