Coding

Part:BBa_K4657001

Designed by: Ying Zhang   Group: iGEM23_BJWZ-China   (2023-10-05)


Coding sequence of ccdB.

ccdB encodes toxic protein (CcdB), which acts as a DNA gyrase toxicant, locking the DNA gyrase together with broken double-stranded DNA, ultimately promoting bacterial suicide. In our initial design, this gene has been optimized and adapted to our chassis strain E. coli MG1655 at the time of synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None