Primer

Part:BBa_K4649012

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-11)


KS Primer

Designed primer specific to genes inserted at multiple cloning site. Used pBluescript vector pBBR1MCS-2, a mobilizable shuttle and expression vector that can replicate in many Gram-negative bacteria such as E. coli and R. tropici.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None