Part:BBa_K4649004
pstB codon optimized for Rhizobium tropici
pstB acts as an ATP hydrolyzing protein that energizes the phosphate transport for the pstABC transporter. It is important because it enables the uptake of phosphate by providing the energy that allows flow through ion channel in the cell membrane through which inorganic phosphate can pass. We designed this part based on research from literature, such as this paper https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1445-3 which further documents the well known pstSCAB system which is known to increase phosphate uptake. We then codon optimized this part for rhizobia, our chassis of choice. We obtained this DNA from Integrated DNA Technologies. We did not encounter any problems with obtaining and using this part. This part can be use din biosafety level laboratory. We recommend that this part be tested in rhizobia, since it is codon optimized for rhizobia compatibility, but it can be modified to work in any bacterial strain. To test this part, we ran a pcr with primers specific to this part, and ran a gel. Since the gel yielded positive results, we know that this part was successfully expressed. During the gel electrophoresis we used a standard negative control to compare the results of the primers.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
Illegal NgoMIV site found at 726 - 1000COMPATIBLE WITH RFC[1000]
| None |