Part:BBa_K4649003
pstA codon optimized for Rhizobium tropici
pstA, along with pstC, form membrane integral proteins that form a membrane channel in the periplasm of a bacteria, allowing for transport of phosphate into the cell. It is important because it enables the uptake of phosphate by creating an ion channel in the cell membrane through which inorganic phosphate can pass. We designed this part based on research from literature, such as this paper https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1445-3 which further documents the well known pstSCAB system which is known to increase phosphate uptake. We then codon optimized this part for rhizobia, our chassis of choice. We obtained this DNA from Integrated DNA Technologies. We did not encounter any problems with obtaining and using this part. This part can be use din biosafety level laboratory. We recommend that this part be tested in rhizobia, since it is codon optimized for rhizobia compatibility, but it can be modified to work in any bacterial strain. To test this part, we ran a pcr with primers specific to this part, and ran a gel. Since the gel yielded positive results, we know that this part was successfully expressed. During the gel electrophoresis we used a standard negative control to compare the results of the primers.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 487
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 487
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 140
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 487
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 487
Illegal NgoMIV site found at 68
Illegal NgoMIV site found at 250
Illegal NgoMIV site found at 310
Illegal NgoMIV site found at 808 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 670
Illegal SapI site found at 189
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