Part:BBa_K4649002
pstS codon optimized for Rhizobium tropici
pstS is the inorganic phosphate binding protein associated with the pstABC transporter. It is important because it enables the uptake of phosphate by bonding to inorganic phosphate and allowing entry through ion channel, and can be used to increase the uptake of inorganic phosphate. We designed this part based on research from literature, such as this paper https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1445-3 which further documents the well known pstSCAB system which is known to increase phosphate uptake. We then codon optimized this part for rhizobia, our chassis of choice. We obtained this DNA from Integrated DNA Technologies. We did not encounter any problems with obtaining and using this part. This part can be use din biosafety level laboratory. We recommend that this part be tested in rhizobia, since it is codon optimized for rhizobia compatibility, but it can be modified to work in any bacterial strain. To test this part, we ran a pcr with primers specific to this part, and ran a gel. Since the gel yielded positive results, we know that this part was successfully expressed. During the gel electrophoresis we used a standard negative control to compare the results of the primers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 94
Illegal AgeI site found at 114 - 1000COMPATIBLE WITH RFC[1000]
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