Part:BBa_K4649001
gcd codon optimized for Rhizobium tropici
The gcd gene codes for the enzyme glucose dehydrogenase (GDH). GDH is part of a direct oxidation pathway with enzymatic conversion of glucose to gluconic acid. It is present in many nitrogen-fixating rhizobia that have been noted for plant growth promotion by solubilization soil soil-bound phosphate in the rhizosphere via the synthesis of gluconic acid under the control of pyrroloquinoline quinone (Pqq). We found mentions of Pqq in the literature regarding phosphate remediation and decided to investigate. To utilize GDH, we decided to overexpress this gene in our R. tropici through Gibson cloning and Q5 assembly. Using UniProt, we found gcd genes from E. coli and codon-optimized them for our R. tropici. However, we encountered issues with creating/receiving the genes from a collaborator due to the gcd genes' high GC content. So, we split the gcd genes into two fragments that we would assemble together in our plasmid. Any biosafety lab can use this gene.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1203
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1203
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1203
Illegal BamHI site found at 1152 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1203
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1203
Illegal NgoMIV site found at 136
Illegal NgoMIV site found at 412
Illegal NgoMIV site found at 465
Illegal NgoMIV site found at 504
Illegal NgoMIV site found at 1998
Illegal NgoMIV site found at 2155
Illegal NgoMIV site found at 2240 - 1000COMPATIBLE WITH RFC[1000]
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