Part:BBa_K4649000

PqqC codon optimized for Rhizobium tropici

Pyrroloquinoline-quinone synthase is one part of the Pqq operon in A. radiobacter. It is important because oxides Ahqq into Pqq, which is the cofactor of glucose dehydrogenase, which solubilizes inorganic phosphate to increase uptake. We researched this part and found it in literature, such as https://www.frontiersin.org/articles/10.3389/fagro.2021.667339/full, about phosphate-solubilizing microbes responsible for plant growth. This paper in specific details the evolutionary function of Pqq via molecular phylogenetics. We obtained this part from IDT. You can use this part in any biosafety level laboratory. This part was codon optimized for rhizobia, so it is best suited for transformation into rhizobia. However, it can be codon optimized for any other bacterial species. To test this part, we ran a pcr with primers specific to this part, and ran a gel. Since the gel yielded positive results, we know that this part was successfully expressed. During the gel electrophoresis we used a standard negative control to compare the results of the primers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 364
  
• 1000
  COMPATIBLE WITH RFC[1000]