Composite

Part:BBa_K4647030

Designed by: Bushra Islam   Group: iGEM23_Stony-Brook   (2023-10-12)


nif KEN with IVA overhangs

Nif gene chunk with nifK, nifE, and nifN assembled with IVA overhangs, which can be used in combination with nif BHD and nif XAV to assemble minimal nitrogen fixation gene cluster for the expression of nitrogenase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 3447
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1136
    Illegal SapI.rc site found at 1519


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