Composite

Part:BBa_K4647029

Designed by: Bushra Islam   Group: iGEM23_Stony-Brook   (2023-10-12)


nif BHD with IVA overhangs

Nif gene chunk with nifB, nifH, and nifD assembled with IVA overhangs, which can be used in combination with nif KEN and nif XAV to assemble minimal nitrogen fixation gene cluster for the expression of nitrogenase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2260


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Parameters
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