Primer

Part:BBa_K4647020

Designed by: Bushra Islam   Group: iGEM23_Stony-Brook   (2023-10-12)


IVA primer designed to anneal to 2BCT and amplify with homology to nifD

IVA primer designed to anneal to 2BCT and amplify with homology to nifD. This primer, with its reverse pair, works to assemble the chunk nifB-nifH-nifD to the plasmid 2Bc-T.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None