Coding

Part:BBa_K4644002

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-09-23)


ureABC

        Providencia rettgeri. UreABC protein coding region.This part is responsible for hydrolyzing urea into carbonate and ammonium ions.

Biology

        Bacterial urease genes were widely reported in a way of urease gene cluster, which was consisted of structural genes and accessory genes. Structural subunits of urease were encoded by structural genes (ureABC), and accessory subunits of urease were encoded by accessory genes (ureEFGD).

Fig 1. Degradation of urea

usage

        We constructed plasmids for PET-lac ure ABC and PET-lac ure EFGD, and connected them through homologous recombination, as shown in the following figure.

Fig 2. PETlac-ureABC-lac-ureEFGD DNA

Characterization

1. Agarose Gel Electrophoresis:

        Urease was extracted from the Providencia rettgeri strain JNB815 and perform PCR replication, and the experimental results were shown in figure 2.

Fig 2. The result of ureABC pcr.

References

Liu, X., Zhang, Q., Zhou, N. et al. Expression of an Acid Urease with Urethanase Activity in E. coli and Analysis of Urease Gene. Mol Biotechnol 59, 84–97 (2017). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1644
    Illegal XbaI site found at 724
    Illegal SpeI site found at 2284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1644
    Illegal SpeI site found at 2284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1644
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1644
    Illegal XbaI site found at 724
    Illegal SpeI site found at 2284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1644
    Illegal XbaI site found at 724
    Illegal SpeI site found at 2284
    Illegal AgeI site found at 686
  • 1000
    COMPATIBLE WITH RFC[1000]


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