Part:BBa_K4638020

EX-NA-MUT

Synonymous mutated Neuraminidase (NA) of WSN in the signaling sequence of Hemagglutinin (HA)

Usage and Biology

The neuraminidase (NA) of influenza A viruses, a class II glycoprotein with the amino terminus inside the cell and the carboxyl terminus outside is one of two major glycoproteins on the virus surface. It can help the virus to get into cells through endocytosis. The other glycoprotein is Hemagglutinin. Hemagglutinin, often abbreviated as HA, is a crucial protein found on the surface of influenza viruses. The HA protein binds to sialic acid-containing receptors on the host cell surface and mediates the fusion of the viral envelope with the endosomal membrane after receptor-mediated endocytosis. On the surface of Influenza, the expression of HA is often more than the expression of NA. We exchange the expressing signal sequence of these two proteins, hoping to enhance the virus’ ability to TfR1. This composite part is the NA in the signaling sequence of HA, using mutated signaling sequence and synonymous mutated NA protein.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 111
  Illegal PstI site found at 944
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 111
  Illegal PstI site found at 944
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1118
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 111
  Illegal PstI site found at 944
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 111
  Illegal PstI site found at 944
  Illegal NgoMIV site found at 219
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1414