Part:BBa_K4638007

NAS28

Neuraminidase of WSN with lengthened stalk by 28 amino acids

Usage and Biology

The neuraminidase (NA) of influenza A viruses, a class II glycoprotein with the amino terminus inside the cell and the carboxyl terminus outside is one of two major glycoproteins on the virus surface. It can help the virus to get into cells through endocytosis. Deleting this stalk area can help reduce its efficiency of infection while adding more stalk area would enhance the ability of the virus to infect cells. NAS28 is the NA protein whose stalk is lengthened by 28 amino acids.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1348
  Illegal PstI site found at 987
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1348
  Illegal PstI site found at 987
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1161
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1348
  Illegal PstI site found at 987
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1348
  Illegal PstI site found at 987
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Here's an agarose gel electrophoresis that we did, comparing the DNA of the NA protein with the deletion of 24 amino acids to the DNA of the NA protein with the insertion of 28 amino acids.

As can be seen from the figure, the molecular weight of the "delete 24" DNA fragment is smaller than that of the "insert 28" fragment.