Part:BBa_K4638006

NAS14

Neuraminidase of WSN with lengthened stalk by 14 amino acids

Usage and Biology

The neuraminidase (NA) of influenza A viruses, a class II glycoprotein with the amino terminus inside the cell and the carboxyl terminus outside is one of two major glycoproteins on the virus surface. It can help the virus to get into cells through endocytosis. Deleting this stalk area can help reduce its efficiency of infection while adding more stalk area would enhance the ability of the virus to infect cells. NAS14 is the NA protein whose stalk is lengthened by 14 amino acids.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1306
  Illegal PstI site found at 945
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1306
  Illegal PstI site found at 945
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1119
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1306
  Illegal PstI site found at 945
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1306
  Illegal PstI site found at 945
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Here's an agarose gel electrophoresis that we did, comparing the DNA of the NA protein with the deletion of 9 amino acids to the DNA of the NA protein with the insertion of 14 amino acids.

As can be seen from the figure, the molecular weight of the "delete 9" DNA fragment is smaller than that of the "insert 14" fragment.

Also,we did the western blog to compare the wild-type NA protein with the 9 amino acid truncated NA protein and the 14 amino acid lengthened NA protein.But only slight differences may be discernible.