Composite

Part:BBa_K4635007

Designed by: Ruohui Li   Group: iGEM23_NEU-CHINA   (2023-10-12)


pCA24N-csgA-spytag

This section is used to express the fusion protein CsgA-Spytag as one of the components of our project product in vivo functional biofilm. We first carried out crystal violet and Congo red experiments. Please refer to experiment for detailed protocol. https://2023.igem.wiki/neu-china/experiment

crystal-violet-experimental-data.png

Fig.1 Results of Crystal Violet Experiment

congo-red-experimental-data.png

Fig.2 Results of Congo Red Experiment

The comprehensive experimental results showed that the addition of Spytag had little effect on the film-forming ability of chassis bacteria, while Mfp5 had great influence on the secretion of CsgA due to its large molecular weight, which led to a significant decrease in the film-forming capacity of chassis bacteria. In the follow-up, we tested the adhesion of biofilm, the anticorrosion ability of biofilm and the biomineralization test. For more information, see BBa_K4635006


https://parts.igem.org/Part:BBa_K4635006


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None