Composite

Part:BBa_K4635005

Designed by: Ruohui Li   Group: iGEM23_NEU-CHINA   (2023-10-12)


pCA24N-csgA-mfp5

This part is used to express the fusion protein CsgA-Mfp5, which is mainly used to verify the idea of enhancing the adhesion of biofilm.

We started with crystal violet and Congo red experiments, see BBa_4635007for more information.


https://parts.igem.org/Part:BBa_K4635007


The experimental results showed that the fusion protein CsgA-Mfp5 had a great influence on the formation of Escherichia coli biofilm, and its effect was far less than that of BBa-4635007. Therefore, we decided to abandon it as the raw material for preparing BMCP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None