Composite
Part:BBa_K4635005
Designed by: Ruohui Li Group: iGEM23_NEU-CHINA (2023-10-12)
pCA24N-csgA-mfp5
This part is used to express the fusion protein CsgA-Mfp5, which is mainly used to verify the idea of enhancing the adhesion of biofilm.
We started with crystal violet and Congo red experiments, see BBa_4635007for more information.
https://parts.igem.org/Part:BBa_K4635007
The experimental results showed that the fusion protein CsgA-Mfp5 had a great influence on the formation of Escherichia coli biofilm, and its effect was far less than that of BBa-4635007. Therefore, we decided to abandon it as the raw material for preparing BMCP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 371
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 371
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 371
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 371
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |