Coding

Part:BBa_K4635002

Designed by: Ruohui Li   Group: iGEM23_NEU-CHINA   (2023-10-11)


spytag

Polypeptide fragments Spytag and Spycatcher have been proven to form hetero-peptide bonds spontaneously in vitro, realizing the binding of two polypeptide fragments.In vitro linkage of two proteins can be realized by spytag-spycatcher.

We obtained spytag fragment by primer with spytag sequence (synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd.).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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