Coding

Part:BBa_K4635000

Designed by: Ruohui Li   Group: iGEM23_NEU-CHINA   (2023-10-11)


csgA from E.coli MG1655

Escherichia coli is an indispensable gene for biofilm formation, which can express CsgA protein which constitutes biofilm. We amplified a large number of csgA gene fragments from the genome of Escherichia coli MG1655 using PCR technology.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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