Plasmid

Part:BBa_K4630119

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-12)


pET28a-eGFP-stgframe

It is the integrated plasmid using green fluorescence to visualise the expected editing of the stgRNA. The specificity determining sequence of stgRNA can be easily changed by Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 912
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1220
    Illegal BamHI site found at 903
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1154
    Illegal SpeI site found at 912
    Illegal NgoMIV site found at 1252
    Illegal NgoMIV site found at 2840
    Illegal NgoMIV site found at 3000
    Illegal NgoMIV site found at 6051
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3920


[edit]
Categories
Parameters
None