Plasmid

Part:BBa_K4630118

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-12)


pET28a-eGFP-stgRNA1

It is the integrated plasmid using green fluorescence to visualise the expected editing of the stgRNA 1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1152
    Illegal SpeI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 912
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1218
    Illegal BamHI site found at 903
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1152
    Illegal SpeI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1152
    Illegal SpeI site found at 912
    Illegal NgoMIV site found at 1250
    Illegal NgoMIV site found at 2838
    Illegal NgoMIV site found at 2998
    Illegal NgoMIV site found at 6049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3918


[edit]
Categories
Parameters
None