Plasmid

Part:BBa_K4630115

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


pCDF-Duet1-T7-stgRNA-cassette 3

StgRNA cassette 3 is under the control of T7 promoter on pCDF-Duet1 plasmid. It is part of our proof-of-concept to verify the function of the cassette recorder under different promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1519
    Illegal NheI site found at 2953
    Illegal SpeI site found at 1511
    Illegal NotI site found at 1536
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1519
    Illegal BglII site found at 1692
    Illegal XhoI site found at 1741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
    Illegal NgoMIV site found at 1711
    Illegal NgoMIV site found at 2206
    Illegal AgeI site found at 1953
  • 1000
    COMPATIBLE WITH RFC[1000]


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