DNA

Part:BBa_K4630103

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-11)


stgRNA-barcode-cassette (0+2)

This part is the combination of the knock-out scar of stgRNA-barcode-cassette 1 (BBa_K4630100) and stgRNA-barcode-cassette 2 (BBa_K4630101). It simulates the situation of the second editing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None