Coding

Part:BBa_K4630006

Designed by: Shuangwu Wu   Group: iGEM23_WHU-China   (2023-10-10)


Cas9 in pCas plasmid

If you transcript and translate the coding sequence, you can obtain a Cas9 protein in you system, which we use in CRISPR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1344
    Illegal NheI site found at 1103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1344
    Illegal BamHI site found at 3382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1344
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None