Part:BBa_K4624614

Laccase B4+B5 Reverse primer for GoldenBraid 2.0 cloning.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

This part was designed for the addition of 3' appropriate overhangs to the lcc2 laccase where the signal peptide has been removed (BBa_K4624004). This part is meant to be used with Laccase B4(+B5) Forward primer (BBa_K4624612). The resulting part will compatible with the B4+B5 position of the GoldenBraid 2.0 cloning grammar.

Figure 1: Gel electrophoresis of the PCR product for the (7) Laccase B4+B5 part with primers BBa_K4624612 and BBa_K4624614. Expected bands (bp): Lane 4: 1538, L=1kb plus DNA ladder.

The successful addition of the appropriate 3' and 5' overhangs was confirmed upon the succesful insertion of the part into the universal part domesticator plasmid pUPD2, which is compatible only with domesticated parts, such as the PCR product.