Primer
Part:BBa_K4624610
Designed by: Christina Malamou Group: iGEM23_Thessaly (2023-09-28)
accCD B4 Reverse primer for GoldenBraid 2.0 cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
This part was designed for the addition of 3' appropriate overhangs to final two subunits (C and D) of the acetyl-CoA carboxylase (accABCD) compatible with the 5' of the B4 position of the GoldenBraid 2.0 cloning grammar. This part is meant to be used with accCD B4(+B5) Forward primer (BBa_K4624609).
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Categories
Parameters
None |