Part:BBa_K4624606

FapR B3+B4+(B5) Forward primer for GoldenBraid 2.0 cloning.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

This part was designed for the addition of 5' appropriate overhangs to FapR (BBa_K4624003) compatible with the 5' of the B3 position of the GoldenBraid 2.0 cloning grammar. This part is meant to be used with FapR B3+B4 Reverse primer (BBa_K4624607) or FapR B3+B4+B5 Reverse primer (BBa_K4624608) depending on the desired final syntax.

Below, we included the results of the gel electrophoresion of the PCR products. For the (3) FapR B3+B4 the primers BBa_K4624606 and BBa_K4624607 were used, while for (4) the primers BBa_K4624606 and (BBa_K4624608) were used.

Figure 1: Gel electrophoresis of the PCR products for the (3) FapR B3+B4 and (4) FapR B3+B4+B5. Expected bands (bp): Lane 3: 601 Lane 4: 602, L= 1kb plus DNA ladder

The successful addition of the appropriate 3' and 5' overhangs was confirmed upon the succesful insertion of the two parts into the universal part domesticator plasmid pUPD2, which is compatible only with domesticated parts, such as our PCR products.