Composite

Part:BBa_K4623021

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)


Twisted Silinker-GFP expression pathway（T7）

Usage and Biology

This composite part is the T7 expression pathway of Twisted Silinker-GFP (BBa_K4623010), which is expressed Twisted Silinker-GFP upon IPTG induction. For more information on Twisted Silinker-GFP, go to BBa_K4623010


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1090
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 1786
    Illegal EcoRI site found at 2008
    Illegal PstI site found at 1103
    Illegal PstI site found at 1729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1090
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 1786
    Illegal EcoRI site found at 2008
    Illegal PstI site found at 1103
    Illegal PstI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1090
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 1786
    Illegal EcoRI site found at 2008
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1090
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 1786
    Illegal EcoRI site found at 2008
    Illegal PstI site found at 1103
    Illegal PstI site found at 1729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1090
    Illegal EcoRI site found at 1618
    Illegal EcoRI site found at 1786
    Illegal EcoRI site found at 2008
    Illegal PstI site found at 1103
    Illegal PstI site found at 1729
    Illegal AgeI site found at 520
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1174
    Illegal SapI.rc site found at 1603


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Categories
Parameters
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