Part:BBa_K4623002

PLGVR

A enzyme cutting site can be cut by MMPs enzyme. The variable peptide segment enables recognition and cleavage by protein cutting enzymes in specific environments, releasing the biotin-modified functional protein that has been cleaved and bound with mSA. To facilitate experimental verification, we have chosen a matrix metalloproteinase 2 (MMP2) cleavable short peptide (PLGVR) as the variable peptide segment. MMPs are a family of zinc-dependent endopeptidases that are overexpressed in the extracellular environment of certain tumors. The linker protein, with PLGVR, can be recognized and cleaved by MMP2, releasing the functional protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References:

[1]Lun Yang, Jian Tang, Hui Yin, Jie Yang, Bin Xu, Yunkun Liu, Zhi Hu, Bentong Yu, Fangfang Xia, and Guowen Zou ACS Biomaterials Science & Engineering 2022 8 (2), 880-892 DOI: 10.1021/acsbiomaterials.1c01418.