Coding

Part:BBa_K4619011

Designed by: Yuqi Liu   Group: iGEM23_UCAS-China   (2023-10-11)


IYRS-MJR1

Full IY-tRNA synthase and amber suppressor tRNA sequence. This part codes for IY( 3-iodo-L-tyrosine) -tRNA synthase, and transcribes into Amber suppressor tRNA, works together with the IY(3-iodo-L-tyrosine)-tRNA synthase to incorporate unnatural amino acid 3-iodo-L-tyrosine in an amber-specific means.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 319
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 319
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 319
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 319
    Illegal NgoMIV site found at 929
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None