Coding

Part:BBa_K4619009

Designed by: Yuqi Liu   Group: iGEM23_UCAS-China   (2023-10-11)


colE3-1amb-IMME3

Codes for the toxin-antitoxin module. Colicin E3(colE3) is a highly toxic RNase which kills host bacterium with a few molecules. 1amb-IMME3 could directly inhibit the activity of Colincin E3 by forming a complex with it. This module works together with IYRS-MYR1, the unnatural amino acid module, to incorporate 3-iodo-L-tyrosine(IY) into the antidote. Therefore, only with foreign supplement of IY can the engineered bacteria stay alive.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
    Illegal AgeI site found at 241
    Illegal AgeI site found at 1507
    Illegal AgeI site found at 1612
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None