Part:BBa_K4619008

CadC-TcpP-p5-pCadBA-Digitalizer-mCherry-Terminator-sRNA-p2

Full TcpP-CadC chimeric receptor and its downstream activation module, which consists of four main components: the TcpP-LBD coding sequence, the CadC-DBD coding sequence, cofactor TcpH, the pCadBA promoter, a constitutive promoter and the digitizer. The assembly process is as follows: We replace the GOI with LuxI in the digitalizer, plug in the TcpP chimeric coding sequence to replace XylS, and add a constitutive expression of TcpH. Regarding the original digitizer, we removed the XylS system, which includes the XylS protein and its corresponding promoter. We then combine these two sections and anticipate that the new chimeric digitizer will function properly. When combined with the primary bile acid, TcpP-CadC dimerize with the help of TcpH, then induce the expression of LuxI under pCadBA, with the control of the digitalizer module.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]