Coding

Part:BBa_K4619002

Designed by: KaMing Li   Group: iGEM23_UCAS-China   (2023-10-09)


LuxRm

Quorum sensing involves the LuxI and LuxR proteins. The LuxI protein generates the quorum-sensing signal 3-oxo-dodecanoyl-HSL(Acyl-HSLs), and then upon reaching a certain concentration threshold AHLs will bind to the LuxR protein, causing the DNA binding domain inside to be exposed. LuxR is a signal-responsive transcriptional regulator that activates the downstream of our pathway by a LuxR dimer binding to an 18-base-pair inverted repeat, the lux box. In our project, we chose the LuxR mutant A1 and the lux box variant A with low background expression and high specific expression to optimize the LuxI-LuxR quorum sensing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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