Part:BBa_K4613891

YF1-PAL-GFP

This composite part contains BBa_K4613429, BBa_K2656009, BBa_4613168, BBa_B0053, BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, and BBa_E0040, which was used as a control to minimize the effect of variables and PAL.

Reference

Ranzani A T, Wehrmann M, Kaiser J, et al. Light-dependent control of bacterial expression at the mRNA level[J]. ACS Synthetic Biology, 2022, 11(10): 3482-3492.
Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature chemical biology, 2019, 15(11): 1085-1092.
Dietler J, Gelfert R, Kaiser J, et al. Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine[J]. Nature Communications, 2022, 13(1): 2618.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 14
Illegal SpeI site found at 103
Illegal PstI site found at 348
Illegal PstI site found at 1017
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 103
Illegal PstI site found at 348
Illegal PstI site found at 1017
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 61
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 14
Illegal SpeI site found at 103
Illegal PstI site found at 348
Illegal PstI site found at 1017
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 14
Illegal SpeI site found at 103
Illegal PstI site found at 348
Illegal PstI site found at 1017
Illegal NgoMIV site found at 1891
Illegal NgoMIV site found at 1963
Illegal NgoMIV site found at 2053
Illegal NgoMIV site found at 2071
Illegal NgoMIV site found at 2583
Illegal NgoMIV site found at 2876
Illegal NgoMIV site found at 2970
Illegal AgeI site found at 393
Illegal AgeI site found at 678
Illegal AgeI site found at 888
Illegal AgeI site found at 999
Illegal AgeI site found at 1605
Illegal AgeI site found at 2751
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2640
Illegal BsaI.rc site found at 1504
Illegal BsaI.rc site found at 4110