DNA

Part:BBa_K4613028

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


Circular Template

Circular Template is a uniquely designed single-strand oligonucleotide. The splint oligonucleotide is annealed to the 5-phosphorylated circular template. The splint oligonucleotide is complementary to short stretches of both the 3' and the 5' end of the linear circular template. T4 DNA ligase then catalyzes the formation of the phosphodiester bond between the 5-phosphorylated end and the free 3 end to form the expected circular template. In our project, we designed circular template as the template of rolling circle amplification(RCA). After Ochratoxin A(OTA) bound to locked hairpin, the RCA primer was released, initiating the rolling circle amplification reaction.

Reference

  1. Zhang J, Lu Y, G, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (ao WOTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None