Tag

Part:BBa_K4613016

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)


SUMO-Tag

In eukaryotic cells, the reversible attachment of small ubiquitin-like modifier (SUMO) protein is a post-translational modification that has been demonstrated to play an important role in various cellular processes. Moreover, it has been found that SUMO as an N-terminal fusion partner enhances functional protein production in prokaryotic and eukaryotic expression systems, based upon significantly improved protein stability and solubility. By construction of a fusion protein, M-CPA, with SUMO-tag, we increased the solube expression rate of M-CPA in Escherichia coli.


Reference

  1. Panavas T, Sanders C, Butt T R.SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems[J].Methods Mol Biol,2009, 497: 303-17.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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