Part:BBa_K4612146

2code_ hsa-let-7a-2-5p

In our experiments, we found that the set of probes we first designed using NUPACK did not work properly(code_ hsa-let-7a-2-5p (BBa_K461240)), so we redesigned them. 2code_ hsa-let-7a-2-5p (BBa_K461246) is a 23 nucleotide single-stranded DNA sequence that is a primary weighted strand of the target hsa-let-7a-2-5p, designed by NUPACK from the target sequence. The primary weight probe of hsa-let-7a-2-5p has its partial complementary sequence on it, positioned after the target (5' to 3'). In order to realize its target hsa-let-7a-2-5p weight number size 25, we use the cascade method to transform 25 into 5*5, so hsa-let-7a-2-5p's first level weight probe combines 5 of this weight chain. The 18 nt at the 5' end of the weighted strand is paired with the weighted probe, and the last 5 nt is not paired with the weighted probe. The presence of this free 5 nt sequence prevents the DNA polymerase from combining with the weighted probe between the weighted strands and initiating a polymerase-mediated strand displacement reaction, resulting in the number of weighted strands displaced, released and less than the corresponding weights of the target.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 13
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]