Part:BBa_K4612145
2hsa-let-7a-2-5p _S1
In our experiments, we found that the set of probes we first designed using NUPACK did not work properly((hsa-let-7a-2-5p _S (BBa_K4612131)), so we redesigned them. 2hsa-let-7a-2-5p _S (BBa_K4612145) is a 117-nucleotide single-stranded DNA sequence that serves as a first-order weighted probe for the target hsa-let-7a-2-5p. The 3' end of the sequence is designed to be complementary to the target hsa-let-7a-2-5p, so that it binds specifically to the target and thus recognizes the input signal. In order to achieve the target hsa-let-7a-2-5p weight number size of 25, we use the cascade method to convert 25 into 5*5, so there are also 5 primary weight strands bound to this probe, and the primary weight strands will be replaced and released by newly synthesized daughter strands during DNA polymerization by Bst DNA polymerase, and the released primary weight strands will be used as a signal to recognize the input signal. The released primary weight strand will be used as the target to undergo polymerase-mediated strand displacement reaction again to realize the multiplication of weights. Through this process, the signal of the target is quantitatively converted into the number of weighted strands, which facilitates subsequent calculations, and finally the results of the operation are converted into different fluorescence intensities, making the results simple and easy to read.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 19
Illegal XhoI site found at 37
Illegal XhoI site found at 55
Illegal XhoI site found at 73 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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