Part:BBa_K4612142
code2_F
code2_F (BBa_K4612142) is a single-stranded DNA sequence with 18 nucleotides and modified at the 3' end with the fluorescent motif BHQ. Together with the corresponding quenching probe code2_Q (BBa_K4612144) it forms a set of reporter probes. This set of reporter probes is used to convert a target signal with a negative weight number into a fluorescent signal after summation. In the absence of the target nucleic acid, the reporter probes bring the distance between the fluorescent molecules and the quenching molecules closer due to the formation of a stable double-stranded structure, resulting in fluorescence resonance energy transfer (FRET) occurring and fluorescence quenching; when the target is added, the weighted strand of the polymerase-mediated strand displacement hybridizes with the long DNA strand in the double-stranded probes through the toehold-mediated strand displacement reaction, resulting in the formation of a thermodynamically more stable double-stranded structure The DNA short chain is also competed down to achieve fluorescence recovery. Since different target DNA sequences have different weights in the previous step, different numbers of weighted strands will be released during DNA polymerization, and therefore different intensities of fluorescence signals will be released in this step of the reaction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |