Part:BBa_K4612136
code_1
code_1 (BBa_K4612136) is a single-stranded DNA sequence with 23 nucleotides, and is a universal weighted strand of positive weight number. The weighted probe has its partial complementary sequence positioned after the target (5' to 3') in an amount determined by the weight number of the target. The 18 nt at the 5' end of the weighted strand is paired with the weighted probe, and the last 5 nt is not paired with the weighted probe. The presence of this free 5 nt sequence prevents the DNA polymerase from combining with the weighted probe between the weighted strands and initiating a polymerase-mediated strand displacement reaction, resulting in the number of weighted strands displaced, released and less than the corresponding weights of the target. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |